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Download e-book for iPad: Affinity Chromatography and Biological Recognition by Irwin M. Chaiken

By Irwin M. Chaiken

ISBN-10: 0121665801

ISBN-13: 9780121665807

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Extra info for Affinity Chromatography and Biological Recognition

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In this mobile receptor hypothesis (Fig. 1) we proposed that receptors which normally modify adenylate cyclase need not necessarily be structural components of this enzyme complex. In fact, it would be AFFINITY CHROMATOGRAPHY AND BIOLOGICAL RECOGNITION 29 Copyright © 1983 by Academic Press, Inc. All rights of reproduction in any form reserved. ISBN 0-12-166580-1 30 PEDRO CUATRECASAS ATP FIGURE cAMP 1 wiser to think of all participating macromolecules as being sep­ arate entities. , adenylate cyclase), thus selectively modifying its activity.

And Richards, F. , Academic Press, New York, pp. 251-451(1976). 3. , The Mobile Receptor Hypothesis and Polypeptide Hormone Action. , Marcel-Dekker, New York, in press. 4. , Developing Concepts in Receptor Research. Therapeutic Frontiers Lecture presented at Annual Meeting of Am. Col. Clin. , Kansas City, June 1982. Drug Intelligence and Clinical Pharmacy', 17,357-366 (1983). PEDRO CUATRECASAS 40 5. , Coupling of hormone receptors to adenylate cyclase of different cells by cell fusion. Nature, 268,310-313 (1977).

In other separate studies, the cells which have been cultured over many generations have been found to become resistant to EGF even though the number of binding sites and their affinity is not significantly affected. If anti-EGF antibody is added together with minute quantities of EGF, very significant enhancement of the response can be obtained (16). Furthermore and perhaps more importantly, the EGF response can be elicited with concentrations of EGF at least 3 orders of magnitude lower than those required in the absence of the antibody.

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Affinity Chromatography and Biological Recognition by Irwin M. Chaiken


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